DC Region AAV User Meeting & Workshop

Join us in Rockville on May 27th!

AAV Workshop: An introduction to the characterization of AAV by light scattering

Adeno-associated virus (AAV) is an attractive delivery vehicle in gene therapy attributed to its mild immune response and ability to deliver the therapeutic genome into a wide range of host cells. To ensure the safety and efficacy of the viral vector-based drug product, it is essential to implement robust and reliable characterization tools throughout the production and manufacturing process. In this presentation, we will discuss a size exclusion chromatography (SEC) method coupled with UV, multi-angle light scattering (MALS), and differential refractive index (dRI) detectors to measure the following three important AAV quality attributes (QAs): 1) total number of viral capsid particles; 2) relative capsid content (e.g., ratio of empty and full capsids); and 3) percentage of monomer or aggregates. Additionally, orthogonal methods using dynamic light scattering (DLS) and field flow fractionation (FFF) will be compared.

AAV User Meeting: Secrets to the successful quantification of AAV by light scattering

We will provide step-by-step training on how to use SEC-UV-MALS-dRI, protein conjugate analysis in ASTRA software, and additional calculations to measure AAV quality attributes (QAs). The QAs include: 1) total number of viral capsid particles; 2) relative capsid content (e.g., ratio of empty and full capsids); and 3) percentage of monomer or aggregates. We will also be available to answer any of your questions regarding DLS and FFF applications and analysis.

Check back soon for Speakers and Agenda!
Details: Date: May 27, 2020
AAV Workshop: 8:30 AM - 11:00 AM
AAV User Meeting: 11:00 AM - 4:15 PM
Location: Hilton Washington DC/Rockville
1750 Rockville Pike
Rockville, MD 20852
Who should attend:
  • Workshop: Anyone who is interested in tools to characterize and quantify AAV and other viral vectors for gene therapy.

  • User Meeting: Active SEC-MALS users who want to learn how to characterize and quantify QAs of AAV particles and other viral vectors.
Check back soon for Speakers and Agenda!

September 2019 User Meeting was held in Bethesda, MD

 
 

September 2018 User Meeting was held in Bethesda, MD

Speakers
  • Ashwinkumar Bhirde, FDA\CDER\OPQ\OBP\DBRR2
    "Innovative Way of Screening Therapeutic Proteins for In-use Stability"

  • Allen Minton, NIDDK/NIH
    "High Throughput Quantitative Characterization of Thermodynamic & Colloidal Stability of Proteins with the DynaPro Plate Reader"

  • Andre Striegel, National Institute of Standards and Technology
    "Multi-Detector Hollow-Fiber Flow Field-Flow Fractionation (HF5) of Polysaccharides"


September 2017 User Meeting was held in Gaithersburg, MD

Speakers
  • Ashwinkumar Bhirde, FDA/CDER
    “High-throughput Screening of Colloidal Nanoparticles Under Simulated Physiological and Therapeutic Conditions”

  • Arun Parupudi, MedImmune Inc.
    “Light Scattering an Indispensable Technique for the Biopharmaceutical Industry”


April 2014 Meeting was held in Gaithersburg, MD

Speakers
  • Lisa Kueltzo, NIH/NIAID Vaccine Research Center
    "Dynamic Light Scattering in Formulation Screening: Applications and Challenges"

  • Chris Rowe, NIH/NIAID Div. of Intramural Research
    "SEC-MALS-DLS for Malaria Conjugate Vaccines: From Bench to Clinic"

  • Allen Minton, NIH/NIDDK Lab. of Biochemistry and Genetics
    "Using DLS to Detect and Quantify the Effect of Osmolytes on Proteins Associations"

  • Di Wu, NIH/NIDDK Lab. of Biochemistry and Genetics
    "Using SLS to Detect and Quantify Nonspecific Protein-Protein Interactions"

  • Reza Esfandiary, MedImmune/Dept. of Formulation Sciences
    "Application of Light Scattering Techniques in Studying Protein-Protein Interactions: A Monoclonal Antibody Case Study"

  • Dan Some, Wyatt Technology
    "ADC Characterization: Molar Mass, Size, Conjugation and Aggregation" and
    "CG-MALS Case Study and How-To: Multi-Valent Protein Ligand Binding"

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