Field-Flow Fractionation

Eclipse DualTec

Unprecedented productivity, and flexibility for separating proteins, polymers, colloids and nanoparticles.

The Eclipse™ DualTec supports both Hollow-Fiber Flow-FFF (HF5) and Asymmetric Flow FFF (AF4) in one instrument. Utilizing one HPLC stack and one set of Wyatt detectors, the DualTec permits automated switching between any two of SEC, AF4 and HF5 separation followed by MALS and/or DLS characterization.

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Eclipse AF4

A versatile system for separating macromolecules, proteins, colloids, and nanoparticles.

The Eclipse™ AF4 is optimal for all standard asymmetric-flow field-flow fractionation (AF4) separations as well as frit-inlet channels and semi-preparative AF4. Combined with industry-leading HPLC equipment and Wyatt's unique multi-angle light scattering detectors, the Eclipse AF4 excels at characterizing difficult samples.

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FFF System Comparison

Capabilities Eclipse™ DualTec™ Eclipse™ AF4
Channel Options
Flat Channels (AF4)
  • Short SC: 153 mm e.l.
  • Long LC: 246 mm e.l.
  • Short SC: 153 mm e.l.
  • Long LC: 246 mm e.l.
  • Semi-Prep SP3: 246 mm e.l.
  • Frit-inlet Channel (FI-AF4)
Hollow-Fiber Channels (HF5) Disposable, ready-to-use hollow-fiber cartridges: 400 µm radius, PES membrane 10 kDa cutoff, channel volume 90 µL No
Switching Automatic switching between two separation modes Optional module for switching between two separation modes
Metal-free version Yes No
Organic version Yes Yes