ASTRA®

Luminary software for macromolecular and nanoparticle separation and characterization
 

ASTRA

Turn your data into results with the ultimate in light scattering software—ASTRA.

The most powerful and versatile software available for the characterization of macromolecules and nanoparticles via multi-angle and dynamic light scattering.

  • Maximize your productivity via ASTRA's One-Click MW™ feature, automated multi-sequence processing and HPLC integration.
  • Take advantage of its integrated control of Agilent systems, or its support for Waters Empower sequences.

ASTRA offers a suite of basic and advanced analyses, including customized reports and an optional 21 CFR Part 11 compliant security and database package.

It greatly simplifies MALS and DLS analysis, so you can quickly and accurately assess molar mass, size, conformation, conjugation and other essential physical parameters.

With each analysis, ASTRA provides the quantitative experimental uncertainties that transform mere numbers—into science.


ASTRA Features and Capabilities

Browse these tabs to learn about the many capabilities and features in ASTRA which make it is the most powerful and versatile software available for the characterization of macromolecules and nanoparticles via multi-angle and dynamic light scattering.

One-Click MW™

One-Click MW

Don't waste time - from sample to results in one click. Read more...

 


One Click: From Sample to Results

One-click MW

 
ASTRA increases productivity for routine analyses

Running a SEC-MALS analyses has never been easier. ASTRA’s One-Click MW™ feature complete an entire method at the click of a button.

Click on Run Default and ASTRA will:

  • Synchronize data collection with your HPLC
  • Autoprocess data to determine MW and radius
  • Generate custom reports and graphs
  • Prepare for the next run

run-default-button

Method Builder

ASTRA's Method Builder simplifies the specification of methods for the most common protein and polymer analyses, in three intuitive steps:

  • Choose your scientific application
  • Specify the instruments
  • Enter sample and run parameters

Then save the method, load the sample(s), click Run Default and return later to find out the MW results in the final report.

Freedom to Explore

With ASTRA, you are never locked in to default methods or standard procedures. You always* have access to each parameter and processing step to review, validate and improve.

*Except network accounts where access is limited under the 21 CFR Part 11 compliant Security Pack.

Ahead of the Curve

As you build expertise and confidence, and move on to complex analyses, ASTRA allows you to refine the results of automated processing with fine adjustments to peak selection, baseline specification, detector filtering and other settings.


A Complete SEC-MALS Solution

Full digital synchronization between your HPLC system, Wyatt detectors and ASTRA:

  • Minimizes user errors
  • Runs SEC-MALS from a single, familiar environment
  • Saves you time and money

ASTRA's HPLC CONNECT module works with standard Agilent HPLC hardware including pumps, autosamplers, column ovens, UV/Vis detectors, and fraction collectors.

HPLC Manager

Integrated HPLC control with HPLC CONNECT

Let ASTRA take control of your HPLC stack for effortless synchronization between injections and data collection. ASTRA eliminates the need for separate HPLC and light scattering software. HPLC modules are listed together with Wyatt detectors in a common experiment configuration, and key parameters such as vial position, injection volume and flow rate are specified in ASTRA's sequences.


Digital signal acquisition

HPLC CONNECT controls UV/VIS detectors directly within ASTRA. Set UV parameters and acquire all signals digitally, without any external cabling required. Select up to two UV channels for analysis – even post-collection.

HPLC Manager

HPLC CONNECT: Your Dashboard

The HPLC DASHBOARD application controls and monitors HPLC modules including pumps, column ovens, UV detectors and autosamplers, right from the desktop. Included at no additional cost with HPLC CONNECT.


Processing with Ease

Built from the ground up to handle large sets of data with ease, ASTRA has the tools you need to process and report on sequences containing many samples, including replicates. The "One-to-Many" batch processing feature lets you focus on your goals, and leave the drudgery to the computer.

EASI Table

Everything you need at a glance.

Quickly compare multiple data runs with the EASI Table feature to assess analyte stability and consistency. The table data can be exported in a variety of formats, or copied and pasted into your favorite word processing or spreadsheet software.

Fully customizable.

Specify which data should be displayed in EASI Table, as well as the format and precision used to display the values. Choose from percent or numerical uncertainties, or omit them entirely.

EASI Table

Sequence Processing

Collect data while you sleep.

Sequences are used to specify and control the automated collection of data as well as system functions. A sequence can be configured with the default method or a different method can be specified for each sample. Sequences also perform post-collection maintenance, such as running the COMET flow cell cleaner, switching the UV lamp off or switching the Orbit to solvent recycle mode.

Sequence

In the Samples view, it is possible to modify sample parameters on-the-fly, adding samples, changing collection times, or skipping samples.


Let your computer do the work.

ASTRA allows you to process an experiment as you wish, then instruct the computer to apply the same settings and analysis steps on any number of additional data files. You can quickly and easily specify which elements of your 'source' experiment should be incorporated in the target experiments.

Processing

Once the processing is finished, your resulting data files are fully analyzed and ready to be reviewed.


Unmatched Analysis

ASTRA Main Screen

Building on over 40 years of light scattering research and development, ASTRA includes an unrivaled range of analysis features. ASTRA takes full advantage of modern multicore computers, processing data at high speed and with superior accuracy. It is available in 64-bit and 32-bit versions.

Molar Mass Determination

Molar Mass via Light Scattering

Light scattering stands alone in its ability to measure the molar mass of macromolecules in solution both accurately and quickly.
Learn more

Distributions

Molar mass and size are determined across peaks in a chromatography run, and moments such as number- weight- and z-average are calculated. ASTRA also supports visualizing the distribution of these measured quantities. This is extremely powerful in assessing how much of a sample falls with a particular range of molar mass or size. Learn more

Protein Conjugate and Copolymer Analysis

If you have an RI detector, a UV detector, and a light scattering instrument, you can directly measure the molar mass, size, and relative fractions of copolymers and protein conjugates. Learn more

Viral Vector Analysis

The powerful combination of SEC-MALS-UV-RI with ASTRA enables the determination of three critical quality attributes in a single assay: particle concentration, capsid content and aggregation.
Learn more

Particles and Number Density

It is possible to measure the size and number density of a sample with just a light scattering instrument. ASTRA supports several sophisticated models to determine the size of a macromolecule based solely on the angular variation in the light scattering intensity.
Learn more

Molecular Conformation

The shape of molecules can be assessed from the molar mass and rms radius of the sample. Learn more

Branching

ASTRA includes sophisticated branching calculations to assess the branching characteristics of a polymer sample. Learn more

Viscometry

ASTRA provides several analysis features that take advantage of differential viscosity data acquired with a ViscoStar™. Determine Mark-Houwink-Sakurada terms, hydrodynamic radius, and more. Learn more

Molar Mass via Conventional and Universal Calibration

Build a bridge between legacy conventional and universal calibration results and modern, accurate light scattering analysis. Learn more

On-Line DLS

With one of the options for online DLS installed, you can extend your data analysis to determine the translational diffusion and corresponding hydrodynamic radius of your samples. Learn more

DLS batch: Cumulants and Regularization

Determine translational diffusion and corresponding hydrodynamic radius of your unfractionated samples. Learn more

Second Virial Coefficient (A2) Measurements

The second virial coefficient (A2) is a measure of macromolecular self-interaction and solute-solvent interactions, and is one of the few parameters that can be used to predict the crystallization properties of a protein. Learn more

dn/dc and UV extinction coefficient determination

While dn/dc and UV extinction coefficients are documented for many existing compounds, they must be experimentally determined for novel samples. ASTRA provides analysis features to measure these critical sample characteristics. Learn more

Absorption Correction

If a sample absorbs light at the MALS laser wavelength, molar mass calculations will be skewed. ASTRA uses the Forward Monitor detector signal (available in all Wyatt MALS instruments) to calculate the laser attenuation produced by sample absorption and correct the scattering signal levels. In this manner accurate molecular weights are ensured, even in the presence of absorption. Learn more...

Band Broadening Correction

ASTRA's robust band broadening correction accounts for interdetector dispersion effects to give you most accurate results possible. Learn more

Baseline Subtraction

Certain analysis techniques, such as FFF or ion-exchange chromatography, cause large changes in refractive index and viscosity measurements as flow rates are modified during the run. This can make it difficult to properly analyze the file, as the RI and viscosity changes due to flow and pressure changes mask those caused by the sample. ASTRA provides a mechanism to subtract a data collection run without sample (a 'blank') from a sample run to reveal the sample-specific signal changes. Learn more

Peak Statistics

Analyze the performance of your column and chromatography system with ASTRA’s built-in reports for column plate count, peak width and asymmetry factor. Learn more

Error Analysis

Wyatt Technology software is written by scientists for scientists. ASTRA was designed from the ground up to properly compute and propagate uncertainties to ensure our results are publication-quality. Learn more

Cutting Edge Technology

Wyatt Technology is committed to pushing macromolecular characterization to new levels. Consequently, we have a very strong program to develop intellectual property. Many of the algorithms in ASTRA are on the cutting edge of scientific analysis, and are either patented or have patents pending. See our Patents page for details.


State of the Art Reporting

Take control of your analysis reports with ASTRA's Report Designer. With just a few mouse clicks, adjust which data are displayed and how they are formatted. Add your own logo, incorporate some personal notes, or just pare down the default reports to focus on what you care about.

Report Designer

Customizable Logo and Summary

Personalize your reports.

In addition to the numerical and content selections provided in the Report Designer, ASTRA allows you to specify a logo image, and where it should be displayed. You can specify the title as well as additional summary information or notes that will appear on the report.

Personalized Data Selection

Choose your data. Choose your formatting.

Control the content and presentation of your reports with a few mouse clicks. Any bit of data generated during analysis is now available for you to incorporate into your report, with just a click of the mouse.

 

Report Data Options

Full Numerical Formatting

Choose your display style and precision.

Click on a result, and ASTRA presents a simple, intuitive interface for controlling the numerical precision and display format of the result. Quickly and easily change results from fixed point to scientific notation, increase or decrease the number of significant digits to display, and determine if you wish uncertainties to be displayed as numerical values or percentages.

Formatting Results


Built from the ground up to comply with 21 CFR Part 11, ASTRA with Security Pack makes compliance complete and intuitive.

  21 CFR Part 11 White Paper


Wyatt Software System Requirements

Please see Host Computer Requirements for details of the computer hardware and operating system required by ASTRA.