VISION™

Optimize, perform, and analyze FFF experiments
 

VISION

Intelligent design, operation and analysis for field-flow fractionation

Flatten the learning curve and increase your productivity. Wyatt offers unmatched software tools for designing, performing and analyzing FFF-MALS experiments.

VISION offers a seamless workflow in a few simple steps, starting with developing and optimizing the FFF method in silico, then running a sample sequence, followed by data processing, refining the separation method, reporting and consolidating the data files in projects.

VISION coordinates data collection in ASTRA®, the most powerful and versatile software available for the characterization of macromolecules and nanoparticles via multi-angle and dynamic light scattering.

The brains behind FFF

VISION software is the intelligent human interface to an FFF-MALS system built on Wyatt Technology’s Eclipse™ FFF instrument and DAWN® MALS instrument. VISION comprises two primary modules: VISION DESIGN™ for in silico method design and optimization and VISION RUN™ for running FFF methods.

 


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VISION

fff-mals VISION software is the central hub for your FFF-MALS system built on the Eclipse™ FFF instrument and DAWN® MALS instrument.

VISION DESIGN

fff-malsCombines simplified, rationalized FFF method development with FFF data analysis to optimize separations based on FFF theory.

VISION RUN

fff-malsSeamlessly coordinates the pump and autosampler front-end with the Eclipse, detectors and ASTRA®, and records FFF, electrical and UV signals for diagnostics and analysis in VISION DESIGN and ASTRA.

ASTRA

fff-malsFlagship software for comprehensive characterization of macromolecules and nanoparticles by SEC/FFF-MALS. Acquires, analyzes and reports measurements from multi-angle light scattering, dynamic light scattering, UV/Vis, dRI and differential viscometry detectors. Building on over 40 years of light scattering research, ASTRA includes an unrivaled range of analysis features.


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Effortless method development

fff-malsThe flexibility of FFF allows users to achieve excellent separations for most applications, but in some cases finding the optimal method for the separation run may require tedious trial-and-error.

VISION DESIGN is an indispensable tool for FFF method development which utilizes in-silico, "virtual" experiments based on FFF theory to eliminate most of the time and effort required for FFF method development.

Enter the estimated particle sizes of your sample of interest, select your channel and predict your fractogram based on:

  • Channel-flow rates
  • Cross-flow profiles
  • Spacer heights

The final design can be transferred in a click to VISION RUN to run and test your method.

The results of a physical experiment can then be fed back into VISION DESIGN to further refine the method. Usually a single test run will give VISION DESIGN the information it needs to help you fully optimize your method.


From virtual to method

VISION DESIGN immediately displays the predicted fractogram using fundamental FFF theory to calculate retention times, including the effects of band broadening and dilution of the sample in the FFF channel. It only takes a few minutes to explore the effect of different cross-flow rates and timings to improve resolution and find the best method - all from the comfort of your desk.

The example to the right shows the process for optimizing a nanoparticle separation.

The green experiment is the result of a default method for separating a sample containing three nanoparticle sizes. The dashed green line shows the cross-flow gradient used, and the solid green line is the measured concentration signal.

The blue experiment is the result of simulations tested to improve the resolution. With a modified cross-flow gradient, the peaks are expected to exhibit baseline resolution.


Particle size distributions

An additional benefit of VISION DESIGN is the analysis of fractograms collected in VISION RUN to determine size distributions based on FFF retention time, even without the use of light scattering data.

Determine the hydrodynamic radius (Rh) and its distribution from a calculation based on fundamental FFF theory or a calibration with reference standards.

The graph shows size distributions of a polystyrene latex mix reference standard calibration and comparison with FFF theory.


Electrophoretic mobility and zeta potential

Electrical/asymmetric-flow field-flow fractionation (EAF4) separates by both size and particle charge to determine zeta potential distributions, even of multimodal and polydisperse populations.

VISION calculates zeta potential and electrophoretic mobility from EAF4 measurements made with different applied electrical fields. VISION DESIGN offers fully automated processing plus determination of peak retention time and its shift with the electrical field.


Enter the Cockpit

fff-malsVISION RUN is a sophisticated control center for the Eclipse FFF system for maximum productivity.

VISION RUN uses the ICF instrument control framework for Agilent® modules from the 1260 series. The complete feature sets of autosamplers, isocratic or quaternary pumps, UV detectors, fluorescence detectors and fraction collectors are supported.

The dashboard shows instrument status with instrument configurations, settings, real-time status and signal display and sample sequences.

Status information from the Eclipse instrument’s Health Indicators are displayed; should any warnings come up, you will receive actionable recommendations.


Automated Sample Sequences

Once a sample sequence table is defined, VISION RUN will coordinate FFF operation and MALS analysis methods. Samples can be added to sequences on the fly for maximum convenience.


Host Computer Requirements

Please see Host Computer Requirements for details of the computer hardware required by VISION.