FFF-MALS

Wyatt Eclipse –Flow FFF Next Generation

Wyatt Technology Eclipse raises Flow-FFF-MALS on a new technological level increasing performance and ease of use. Dilution control module DCM and Eclipse Mobility are two exiting new features. FFF is a front-end for light scattering which generates the absolute size distribution from FFF separation. The Eclipse was developed from the start for coupling with Wyatt MALS detectors as an indispensable part of the complete solution.

Flow FFF is like HPLC as the sample is transported in a liquid phase, but instead of a column, separation takes place in the FFF channel. The separation mechanism is based on diffusion in a laminar flow with a cross-flow applied. Flow-management in the FFF channel makes the difference between HPLC and Flow FFF. The best HPLC pumps, autosamplers, spectroscopic detectors and fraction collectors are the best choice for Flow FFF as well.

Therefore the Wyatt solution is to use market leading HPLC from Agilent, adding the Eclipse controller and channel and run it with the specialized software VISION to create a seamless integrated system for Flow FFF.

Wyatt Eclipse Turn-Key System

Wyatt Technology provides a complete solution for Flow FFF-MALS consisting of:

  • Eclipse controller and channels
  • Wyatt Technology’s suite of online detectors for MALS, DLS, RI and IV: DAWN, Optilab and ViscoStar
  • Agilent 1260 Infinity II including pump, autosampler, UV/Vis/DAD and fluorescence detectors, fraction collectors, tailored for the target application
  • VISION Chromatography Software Hub to coordinate three applications:
    • VOYAGER software for FFF control and data acquisition
    • SCOUT software for FFF method development, data visualization and analysis
    • ASTRA software for acquisition and analysis of experimental data Wyatt Technology takes full responsibility for the complete system from delivery to installation, training and support

Nanomedicine

Question, if we want this here, or if there is a link to the application or landing pages?

General

The Eclipse Flow FFF system comes with tools for a streamlined workflow, making it straightforward to develop a separation method, optimizing the method and perform advanced data processing procedures. Not only for the expert, but in a walk-up mode for everyone utilizing predefined methods and sequences.

Starting point is computer aided method development to create a flow program which is capable to separate a given set of sizes. Methods are run in automated sequences to process many samples in an unattended mode. All components of the system are controlled from one place, HPLC, Eclipse controller and ASTRA for MALS data acquisition. Comprehensive data processing capabilities are available to get the most out of the experiment.

Service reminders and extensive log of system parameters help to maintain the system in perfect shape and prevent down time.

Software

All software modules are accessed from one place, the VISION hub. VOYAGER is for sample running, SCOUT for method development and processing. ASTRA for comprehensive light scattering acquisition and processing.

A system check application compiles all relevant data which allows Wyatt support to trouble shoot a possible problem.

Licenses are managed in one place.

Additional resources to learn about the uses of SEC-MALS, e.g. publications and webinars, links to theory pages

Controller

Wyatt Eclipse Controller Overview

The Eclipse controller turns the HPLC to an FFF system. It houses all flow components needed to manage the FFF experiment. Pump, autosampler, the Eclipse channel and the detector chain have fluid connections to the controller side panel. Using a sophisticated, proprietary technology, inside the controller the injection flow, the cross-flow and an optional slot-outlet flow are generated from the main HPLC pump flow. This concept realizes highest precision of all flow rates, minimizes pressure fluctuations and has a compact footprint on the lab bench.

The controller highlights a 10” multi-touch display which allows performing manual operations, such as washing steps, at the instrument. Built-in intelligence provides health indicators and guided user assistance as well as self-diagnostics procedure.

Setup and control is facilitated because the software has access to all those parameters reflecting the system status.

A history of system parameters and error states is kept in the instrument logs which helps in trouble-shooting and maintenance operations. Like all NEON instruments, field serviceability has been a prime design goal.

Controller Details

Wyatt Eclipse Controller with versatile functionality

The Eclipse controller supports all variations of Flow FFF experiments to cover different requirements for the most challenging applications.

Center-downstream injection: for analytical, semi-prep and frit-inlet (AF4) channels. Advantage for sensitive, aggregation prone samples and for large injection volumes.

Tip-injection: for hollow-fiber (HF5) channels and for SEC columns. Makes switching between FFF and SEC straightforward.

Autofocus: the focusing position is measured with a flowmeter and automatically adjusted to the desired position. It makes a visualization of the focus position with a colored sample redundant and takes away the requirement to have a transparent cover plate for the channel.

Dual-channel switching is available as an option and allows to switch between two FFF channels or from FFF to an SEC column. This way the sample can be measured from the same autosampler vial both with FFF and SEC.

Dilution Control Module (DCM): available as an option, increases the sample concentration at the detector by up to a factor of 8. Multi-solvent capability: aqueous and organic solvents can be used with the same Eclipse controller unit.

Center Downstream Injection

Tip Injection

Controller Intelligence

Channels

DCM Overview

DCM Innovation

Mobility

Training, Service and Support: If I get it, how will I manage?